Abstract: With the advent of the era of artificial intelligence, human-machine relationship shows a new form of mutual integration and cooperation. Innovation is an important source of enterprise management and development. Exploring the impact of intelligent machines on employees' innovation behavior after enterprises apply intelligent machines to assist work is an important research issue of current organizational behavior. Based on resource conservation theory and self-determination theory, this study introduces learning goal orientation as an intermediate variable and ethical atmosphere as a moderating variable to explore the path of human-machine relationship affecting employee innovation behavior. Through the empirical study, it is proved that human-machine relationship positively affects employees' innovation behavior; The relationship between learning-goal-oriented mediating human-machine relationship and employee innovation behavior; Ethical atmosphere not only regulates the influence of human-machine relationship on learning goal orientation, but also mediates the role of learning goal orientation. According to the above research conclusions, the article suggests that managers should pay attention to the impact of the introduction of artificial intelligence machine on employees, realize the importance of learning goal-oriented, and pay attention to create a good ethical atmosphere in enterprises.