Abstract: To establish a method for the determination of acrylamide in processed foods by using high performance liquid chromatography tandem mass spectrometry, series of processed food substrates were extracted and purified, and then the target substance was separated and qualitative analyzed with LCMSMS, and the isotopic internal standard quantitative method was also used to determine the qualitative of acrylamide in processed foods. By optimizing the chromatographic condition and mass spectrum parameters, the acrylamide standard solution from 3 ng/mL to 2 000 ng/mL had a good linear relationship (LR)with LCMSMS, the LR≥0.999, and the detection limit was 3 μg/kg, and the quantitative limit was 10 μg/kg and the detection method also had good recovery. The method had good stability and accuracy and good recovery rate. It was suitable for qualitative and quantitative analysis of acrylamide in processed food.