Abstract: The correlation and grey relation analysis was used to analyze the relationship of the soybean isoflavone with main traits of 54 soybean in Heilongjiang province. The accuracy and reliability of the high performance liquid chromatography for determination of soybean isoflavone was valid for the method. The average content of total soybean oflavone was 2.895 μg/mg. The average content of Daidzin was 1.231 μg/mg. The average content of Glycitin was 0.268 μg/mg. The average content of Genistin was 1.397 μg/mg. Five soybean varieties, in which isoflavone content exceeded 4.000 μg/mg, were screened out. This paper provides basis for seed selection, breeding and evaluation isoflavones content in soybean.