马兜铃酸对人血清白蛋白二级结构的影响

Effect of Aristolochic Acid on the Second Structure of Human Serum Albumin

摘要: 采用分子对接技术研究了马兜铃酸与人血清白蛋白的作用模式, 结果显示: 马兜铃酸与人血清白蛋白在亚结构域ⅡA结合, 二者间的作用力主要为疏水作用。利用傅立叶红外光谱和圆二色性光谱技术考察了马兜铃酸对人血清白蛋白二级结构的影响, 结果表明: 马兜铃酸与人血清白蛋白的键合使人血清白蛋白中α-螺旋结构含量从46.6%降到43.6%。

Abstract: The binding mode between aristolochic acid and human serum albumin was studied by molecular docking technique. The results indicate that aristolochic acid binds within the subdomain IIA of HSA, and hydrophobic force plays a main role. In addition, the effect of aristolochic acid on the second structure of human serum albumin was investigated using CD and FTIR techniques. As the results of CD proved, the α-helix contents of HSA decreased from 46.6% to 43.6% after binding with aristolochic acid.