Abstract: To establish the method to distinguish Huangzhi hugan tablet by TLC and determine the content of baicalin by HPLC, coptis chinensis Franch, Gardenia jasminoides Ellis, Schisandra chinensis(Turcz.)Baill, Notopterygium incisum Ting ex H.T.Chang were identified by TLC. The content of baicalin was determined by HPLC. The samples were separated on Diamonsil C18 HPLC column with the mobile phase of methanol-water-glacial acetic acid(50∶50∶1). The flow-rate was 1.0 mL·min^-1 with the colume temperature of 35 ℃. The detection wavelength was 274nm. Feature spots of Coptis chinensis Franch, Gardenia jasminoides Ellis, Schisandra chinensis(Turcz.)Baill. Notopterygium incisum Ting ex H.T.Chang were detected in the thin layer chromatography(TLC). The linear relationship of Baicalin was well between 0.354-3.54 μg(r=0.999 8), the average recovery was 99.77%, RSD was 0.32%. The average content of baicalin in five batches of Huangzhi Hugan Tablet was 3.92 mg·g^-1. The specificity of above experiments was well. The methods can be used to distinguish Huangzhi Hugan Tablet and determinate the content of baicalin.