Negative behavior of leadership is an important cause of employees' work withdrawal behavior. Based on social exchange theory and social cognition theory, this paper explores influence mechanism of non-contingency punishment on employees' withdrawal behavior by constructing a theoretical model with non-contingency punishment as independent variable, leader trust as mediating variable, emotional intelligence as moderating variable, and employees' withdrawal behavior as dependent variable. The results show that: 1) non-contingency punishment has a significant positive effect on employees' withdrawal behavior; 2) leader trust plays a partial mediating role between non-contingent punishment and employees' withdrawal behavior; 3) emotional intelligence can weaken negative effect of non-contingency punishment on trusting leaders; 4) emotional intelligence negatively moderates mediating effect of leader trust on non-contingency punishment and employees' withdrawal behavior. That is, the stronger the employees' emotional intelligence ability, the weaker the mediating effect of leader trust. The study reveals mechanism and boundary conditions of non-contingency punishment on employees' withdrawal behavior, and provides some theoretical reference for leaders to reasonably implement punishment and restrain employees' withdrawal behavior.