Facing the economic downward pressure, efficiency has played a key role in a company's performance. This paper analyses the efficiency of China's listed companies in E-commerce industry. The number of staff, operating costs, and total assets are selected as the inputs whilst prime operating revenue, net profit are chosen as the outputs. This paper based on those indicators evaluates the efficiency of the 26 companies. On the whole, those companies are of good performance in terms of efficiency, of which 9 companies get better efficiency. This paper puts forward some suggestions on improvement for these relative low efficient companies. The data shows that the market has almost been occupied by existing companies, which means that it would be difficult for the new ones. It is suggested that the government should introduce some corresponding policies and measures to promote the agricultural E-commerce to maintain a sustained and sound development.